Spend a little more and you can get an FTDI-based USB cable. Animal modelThe PEG precipitation assay was performed with VRC01-WT mAb at a final protein concentration of 0. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. pH-Dependent Solubility. 3. After the plate is read, data will appear in the plate section: 10. The absorbance at 450 nm was subsequently recorded with SpectraMax M5 plate reader (Molecular Devices). Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. Uncontrolled Copy for Training and Reference Purposes Only . 5. The drawing shows a standard microplate of 15 mm height covered with a lid. One unit of hexokinase is the amount of enzyme that will generate 1. The TRITC and FITC labeling process is conducted as in a previous study . We have run the program successfully on macOS High Sierra using the built-in Terminal and Windows 10 using Anaconda Prompt, processing data from a Molecular Devices SpectraMax M5 plate reader. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Detection Modes. The percent relative binding potency was calculated by dividing the reference material. T o enable accurate quantita-tion, initially standard curves were plotted f or both Pi and. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. 5 hours after an initial 10-minute incubation period. Molecular Devices LLC temperature controlled molecular devices m5 plate reader Temperature Controlled Molecular Devices M5 Plate Reader, supplied by Molecular Devices LLC, used in various techniques. Here, we build on this capability and develop an inducible, self‐propagating transposon platform for continuous genome‐wide mutagenesis and the dynamic. Immunohistochemistry Four μm kidney sections were heated at 65 °C for 1 hr and deparaffinized in xylene, followed by rehydration in decreasing concentrations of ethanol (two washes in 100% ethanol, two washes in 95%, one wash in 70%, one wash in 50%,. You can also interpret your results using integrated data analysis software. In general, reading fluorescence from the top is more sensitive than reading from the bottom. 3) At the bottom of the strip, click on "Show. Power on the Epoch Biotek Gen 5 absorbance microplate reader. SpectraMax Paradigm Multi-Mode Safety Guide fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. I’m. M5 SpectraMax M5e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5e models. For a higher degreeTime Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. plates with an 8-fold dispenser (Bio Tek Germany, Bad Friedrichshall, Germany) and the plates were then centrifuged for 1 min at 1,000 rpm and room temperature. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. The BMW M5 CS is the end of an era, one final swan song for internal combustion M5s, and it sends its era out with a loud, violent, obnoxiously fast bang. Enzyme activity from tissue homogenates was normalized to total protein content with the Bradford protein assay (Biorad). plates in 150 ll OS minimal medium (Schnider-Keel et al. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. 100 μl of 2% EB (Sigma-Aldrich, St. A barcode reader accessory can be purchased with theIf you want to get the most sensitivity, you should set the plate reader excitation and emission wavelengths to different values for the two dyes. The assay was performed on HDL collected. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The plate was read by a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA) at 450 nm with 620 nm as reference. Softmax Pro GxP Software 6. Table of Contents. 25 fmol/well, 384-well plate)−top Fluorescein 4 pM (0. 1. Fluorescence Microplate Readers. Reactions on 384-well plates (Corning) were monitored by fluorescence (emission 545 nm) using a SpectraMax M5 plate reader (Molecular Devices), with excitation at 274 nm, for 20 min at 2-min. View the Molecular Devices website. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 6. com 10. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Information on how to reserve the plate readerMolecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Turn on the SpectraMax M5 Multi-Mode Microplate Reader. Choose monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. 8K members Since 2000 M5Board is the best forum community for information on the BMW M5 E60 (V-10), E39 (V-8), E34. reader. We use the SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA). #3635 or Greiner cat. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch:FlexStation® 3 Multi-Mode Microplate Reader. Product Overview. Information on cell morphology, localization of signal, cell count, and more is obtained with Cytation 5 imaging mode. Preparation of biotin–tyramineThe SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. Standards and QCs are composed of a. Representative fluorescence images of the cell-containing scaffolds and well plate are shown in Fig. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. ScanLater Western Blot Detection Cartridge. Viability Assay reagent (Promega). 3. 흡광, 형광, 발광 중 하나의 측정 모드만 이용할 수. 2 mg/mL using 2 different UV-visible spectrophotometers, a SpectraMax M5 plate reader, and a NanoDrop instrument. Then, cells were treated with TCS of different concentra-tions (10, 20 and 40 μg/mL), according to theThe luminescent signal was quantified in a SpectraMax M5 plate reader (Molecular Devices, San Jose, CA). The degradation. 3. For Western blot analysis of apoptosis marker caspase-3, the equivalent total protein from control and SPM. easy to use and read multiple plates very quickly. 0. Bioluminescence assays showed that the engineered Luc-Cys was fully active when compared with 6 × His-NanoLuc prepared in our previous work (Zhang et al. 5 cm) Weight 80 lbs (36. The IC50 was calculated using the DRC package in the R software program (v3. All values reported represent the mean of at least three independent experiments. Thus, the. The following document is. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Narrow your selection of used microplate readers using the filters above or select the one of the categories on the left to view multi-mode or. Inhibition assays at multiple compound. Signals of wells containing only the target cells. Apoptosis was measured by annexin V–fluorescein isothiocyanate (FITC)/7-amino-actinomycin D (7-AAD) labeling followed by fluorescence flow cytometry as previously described. 0200-0729. Analyst, M5, Flex 3, and SpectraMax L readers work differently. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Fluorescence experiments were carried out using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA). 25 pM (0. All kinetic reads were taken at 25 °C in nonbinding all black clear-bottomed Greiner 96-well plates covered with optically clear films and stirred for 10 s prior to each reading. Our luminescence plate readers, also referred to as luminometers are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer. The patented Hybrid Technology offers flexibility and sensitivity across a broad range of applications. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast to address many applications and workflows. The plate was washed and an anti-rabbit peroxidase-conjugated secondary antibody (Jackson Immunoresearch) was applied at a 1:3000 dilution in PBS. 2. 0 . For a higher . Thank you!. High-performance luminescence, fluorescence, UV-Visible absorbance, BRET and FRET, two-color filtered luminescence and kinetic measurement capabilities. 4. SpectraTest ABS2 Absorbance Validation Package performance tests. AC16 cells were treated with control, 5 mmol/L LiCl or 2. A microplate reader with fluorescence intensity (FI) detection uses a light source, usually a Xenon flash lamp or LED, to excite a fluorophore (fluorescent molecule) at a particular wavelength. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Varian Inova 500 MHz three-channel Z-axis PFG NMR. Cells were seeded at a density of 1,000 cells/well (A549: 500 cells/well). The solution was shaken at 37°C and 1200 rpm for 3 days. The plate reader assay was then repeated in SC-URA media with and without. Immunoblotting. edu, 814-863-3503. CHO-K1 cells were transfected with luciferase. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. The plate was incubated for 4 h and the absorbance of the solubilized formazan was measured on a Molecular Devices Spectramax M5 plate reader at 570 nm. The patented Hybrid Technology offers flexibility and sensitivity across a broad range of applications in the clinical laboratory. Application Area: Multi mode plate reader. Accordingly there are different configurations possible. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. siRNA (750 pmol) was transfected into NRK-49F (1 × 10 6 cells) using a Nucleofector Kit V and the Amaxa Nucleofector. To the OP, if you are familiar with VAG-COM, you know that RS232-based VAG-COM cables are in the $14 range on eBay. DH5α and BL21 (DE3) chemically competent E. The ideal measured bioluminescence range is 10 3 –10 6 counts/well at a SpectraMax M5 plate reader using a white opaque 96-well plate. Learn how to use the SpectraMax M5 SpectraMax M4 SpectraMax M5 SpectraMax M5e Multi-Mode Microplate Reader, a device that can read microplates and cuvettes with. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by issuing a single The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Microplate Reader Shelf. the contents were carefully mixed by pipetting up and down. 9000-0756. 4. GloMax® Discover is a ready-to-use multimode plate reader developed with Promega reagent chemistries to provide a simple means of detecting luminescence, fluorescence and absorbance. Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. Results: In the presence of Cliradex ®, Johnson’s® Baby Shampoo, OCuSOFT Lid Scrub®Before termination, 2 μM of Rhodamine-123 was added in each well and plates were again incubated at 37 °C for 30 min. Luminescence detection typically offers greater sensitivity than absorbance or fluorescence detection. Lysin A constructs were expressed in M. 51 L/g). The software is simple to use and set up protocols. Vials were coded so that the person running the samples was blinded to the contents. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. FilterMax F3 and F5 Multi. Description Figure 1. 5 and 10 nm were used for excitation and emission, respectively. Products : SpectraTest Validation Plates ABS2, SpectraTest Validation Plates ABS1, SpectraTest Validation Plates FL1, SpectraTest Validation Plates LM1. Colorimetric detection of crystal violet released into the supernatant, which is proportional to the number of attached cells, was determined using a SpectraMax M5 plate reader (Molecular Devices). Learn how to use the SpectraMax M5 SpectraMax M4 SpectraMax M5 SpectraMax M5e Multi-Mode Microplate Reader, a device that can read microplates and cuvettes with different modes and settings. Detection modalities. 1, and the color is formed by the reaction in the microtiter plate. The Back Panel Figure 1. About Europe PMC; Preprints in Europe PMCLuminescence values were measured on a SpectraMax M5 plate reader (Molecular Devices). Power tested, slide moves in and out of unit as. Certain functionalities in the table above requires specific configurations. All enzymes used for DNA manipulations were purchased from New. 1. Dimensions 20" D x 16. Also refer to this Multimode Reader Comparison Chart. 313 reference gain check fail. 4 fmol/well, 384-well plate)−bottom Reading Speed (Kinetic) 96 wells: 11 s 384 wells: 22 s Luminescence Wavelength Range 300−700 nm Dynamic Range > 6 decades Sensitivity. The Formazan crystals formed in each well were dissolved by adding 100 µl DMSO. 316 grating motor driver faulty. The EC50 values were calculated from the absorbance values vs. Read Speed. PURPOSE. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing a single SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Enter Sample IDs (HPV-Type, Sample Description and Data Reference when applicable), Dilution Factors, and background information into the template. 9. 7°C (gradient heating design). Published online 2016 Jul 13. OD 600 and luminescence were measured at 30 minute intervals for 48 hours. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Use of Attune Flow Cytometer, Leica Cryostat, M5 Plate Reader, and Typhoon 5 require training; please contact Cell_Bio_Operations at hms. 312 gain calibration failed. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. 306 plate air cal fail,low light: 316 grating motor driver faulty: 307 cuv air ref fail: 317 monitor ADC faultyThe BioTek Cytation 5 cell imaging multimode reader combines automated digital widefield microscopy with conventional multimode microplate reading. Fluorescence measurements were performed on a SpectraMax M5 plate reader with an excitation of 440 nm. Endothelial permeability was also measured in vitro, using a transwell assay. The password is. 3. Extracting viral DNA from 4 x 109 copies of the AAV8 preparation ( nominally 5 ng ssDNA)The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. After 24 h, cells were treated as described for the individual figures, then assays were performed 48 h after transfection. To detect the luminescent signals, 100 μl of each dilution was added into a 96-well plate before adding 50 μl of LumiGLO Peroxidase Chemiluminescent substrate (KPL, 54-71-00) to measure luminescent intensity by a SPECTRAmax M5 plate reader. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. After mixing with freshly diluted substrate (50 μl well −1), bioluminescence was immediately measured on a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA) under luminescence mode. Each plate reader or washer is calibrated, inspected, and lubricated—any potential issues are proactively addressed. 2. Description Figure 1. Mutations in the α2–α2′ and α4–α4′ dimer interfaces affect the secondary structure of the DENV2C protein. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. 1 software to identify the IC50. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Table of Contents. The average 526/470 ratio for the sample aliquots. Devices’ StakMax® Microplate Stacker in a matter of minutes and . 4. 1. A. Molecular Devices Spectramax M5 Microplate Reader; Related products. 025 fmol/well, 384-well plate) Quad monochromator: Fluorescein 2. SpectraTest ABS2 Absorbance Validation Package performance tests. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. 0 adjusted at rt containing 10% v/v DMSO) (100 μL) and a 2- or 5-fold dilution was. xlsx file, with or without the averages and normalizations. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. 19 , 25 Human umbilical cord endothelial cells (ATCC, Manassas, VA) were grown in 24-well plates coated with. 2 MΩ cm. Options include top and bottom detection, temperature control and reagent injector. Category 2 and 3 microplate readers use gradient heating as well. 05 were scored as. between the affordability of filter-based readers and the . 1 M NaCl at varying pH levels (3–10) and incubated at room temperature for 1 h. Then, luminescence of samples was read in a Spectramax M5 plate reader with integration time of 5 s. Documents. 8 cm x 41. 0 μM), and this response was consistent in both plate reader assays and on chip (Supplementary Figure 5d. Following lysis, the plate was centrifuged at 500g for 3 min and supernatants transferred to a Maxisorp 96-Well Flat-Bottom Assay Plate (Nunc). [1] AnkerMake M5 is capable of printing at a speed of 500 mm/s and acceleration of 5,000 mm/s² with the new Fast Mode upgrade. Fluorescence was measured with the SpectraMax M5 plate reader with the same excitation and emission wavelengths as the chemical denaturation assay. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. The cell lysate was then transferred to a white opaque 96-well plate (50 μl/well), mixed with the freshly diluted substrate (50 μl/well), and bioluminescence was immediately measured on a SpectraMax M5 plate reader (Molecular Devices) using the luminescence mode. The peptide concentration was. The measured specific activity of NanoLuc is 1. The resulting OD 426 values are visually represented above as a color map generated in the plate section of SoftMax. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of. 2016 Aug 2; 7(31): 50027–50042. g. 7. The exact concentration of the RNA stock solution was calculated by UV absorption at 260 nm (measured by Spectramax M5 Plate Reader, Molecular Devices, San Jose, CA) with the accepted extinction coefficient of the RNA (21. 01 M HCl was added, and absorbance was read at 570 nm using a Spectramax M5 plate reader (Molecular Devices). After that, DCFH-DA was used to mark cells and the ROS level was determined by Spectramax M5 plate reader and five parallel samples were prepared for each group (Wu et al. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. The plates were centrifuged at the end of incubation, and fluorescent signals in supernatants were measured using a SpectraMax™ M5 microplate reader, with excitation at 485 nm and emission at 520 nm. The plate reader can measure multiple readouts, including UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. edu, 814-863-3503. 2 software for data reduction (Molecular Devices, Sunnyvale, CA). Kinetic measurements were done on a SpectraMax M5 plate reader (Molecular Devices) monitoring absorbance at 380 nm at 22 °C using at least three independent measurements. A reaction volume of 12 µL was placed into each well of 384-well clear bottom black-walled plate that was sealed with an oxygen impermeable membrane. The fluorescence intensity was measured using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, Calif) with excitation/emission at 485/530 nm. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. 2. 6-, 12-, 24-, 48-, 96- and 384-well plates. The medium fluid was, respectively, collected at 4. 25 pM (0. The fluorometric performance of theAbsorbance is read with a SpectraMax M5 plate reader. After cell attachment, culture media was replaced with F-12 media containing 1% FBS for overnight incubation. Figure 1. 9. IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. Luminescence values were measured on a SpectraMax M5 plate reader (Molecular Devices). Fluorescence measurements were performed at 30°C (S30 extract) or 37°C (PURE system) using a SpectraMax M5 Plate Reader (Molecular Devices) set to 610 nm excitation and 650 nm. 3). Our comprehensive multipoint inspection and testing ensures each microplate reader or washer meets our product operational specifications. In my opinion one the best plate readers which I used till now is Molecular Devices SpectraMax M5 Plate reader. HSL_EQ_007: Use and Maintenance of a 2-8°C Refrigerator in the HPV Serology Laboratory 3. Even better, they require MINIMAL modification to. The WT DENV2C and its single-point mutants L50S, L54S, L81N and I88N were. The GloMax® Explorer is a high-performance multimode plate reader that allows you to get up and running quickly, generating the data you need from your experiments. Results: In the presence of Cliradex ®, Johnson’s® Baby Shampoo, OCuSOFT Lid Scrub®were read using SpectraMax M5 plate reader (Molecular Devices, San Jose, CA, USA) with excitation of 380 nm and emission of 460 nm. Published online 2016 Jul 13. 5 × 10 5 counts/fmol when measured with a Spectromax M5 plate reader using white opaque 96-well plates. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. SpectraMax Readers have the most complete level of product validation and compliance. Small molecule ligands that engage the regulatory. Reader Documentation Version No. 1. Simply unpack it, plug it in, and begin your experiments. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. LbL films containing TRITC- or FITC-labeled PAA are degraded by DTT and monitored by a fluorometer (SpectraMax M5 Plate Reader, Molecular Devices). To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. MOLECULAR DEVICES VMax Kinetic Microplate Reader For Sale. After the plate is read, data will appear in the plate section: 11. The eight mammalian Src-family tyrosine kinases are dynamic, multi-domain structures, which adopt distinct “open” and “closed” conformations. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. You can use it for many applications (OD, fluorescence, luminescence). With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. 6 for further experiment, so I'm confused which i would choose for OD value determination. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. The colorimetric reaction was stopped with 2 M sulfuric acid, and absorbance was measured at 450 nm using a SpectraMax M5 plate reader (Molecular Devices). SpectraMax M-series Microplate readers. Please contact Tatiana to reserve the plate reader time: [email protected] signals were scanned using a Molecular Devices SpectraMax M5 plate reader (M5, Molecular Devices, U. 25% (v/v) AlamarBlue reagent was then added to cells and incubated in normal cell culture conditions for 1 h, protected from light. The modular platform allows upgrading to expand functionality as your laboratory workflows change. The sample is typically placed in a cuvette through which light is sent horizontally. Select the lab user profile. [4] The IC 50 value of each drug was calculated by the Logit approach. 4: Test tube cover. Circular Dichroism. The absorbance was measured using Molecular Devices Spectramax M5 plate reader with the software Softmaxpro at 570 and 650 nm. When the user applies a set temperature of 37°C, the top is heated to 37. Viability was evaluated using the Cell Titer Glo Assay (Promega) and luminescence was read on a SpectraMax M5 plate reader (Molecular Devices). HeLa cells were seeded in a 6-well plate with standard DMEMfluorescence intensity in the supernatant was measured in a SpectraMax M5 plate - reader (Molecular Devices) at 485 nm excitation and 535 nm emission wavelengths. Read More. Select Update Driver. com 10. Wide range of applications The VersaMax reader covers a wide range of applications:To monitor the increase rate, fluorescence measurement is then performed at 37°C at 10-s intervals for 10 min with Ex/Em 450/510 nm in the plate reader (Spectramax M5 plate reader, Molecular Devices, For each bacterial sample, we performed the reactions in duplicate and averaged the results. The plates were washed thrice with kit wash buffer; 150 µl kit substrate was then added to each well and the plate incubated 30 min at room temperature (with shaking). 3. Top reading usually provides better signal-to-noise ratios for solution-based assays such as DNA quantification or protein quantification. Microplate Capacity. , Sunnyvale, CA, USA) at the excitation wavelength of 488 nm for carboxy-H2DCFDA and 505 nm for DHE and MitoSOX ™. Cell Viability Assay Using Violet Staining The cells were seeded into 24-well plates and cultured in normal or growth medium or hormone-depleted medium with the relevant treatments as. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. 0 mM of NADH per min at pH 8. 17. 1. PMC full text: Oncotarget. 4 software (Molecular Devices, San Jose, CA, USA). 7. 10569The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. PDE-lux transcriptional fusion plasmids were constructed as previously described. Major items of equipment include the following: Agilent MR400 400 MHz two-channel PFG NMR. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. On microplate readers,. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. Absorbance was measured using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA) at a 540 nm wavelength, and was used to determine the collagen content. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Description Figure 1. Blue, kinase (no inhibitor); green, no kinase. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. 0 and room temperature. The lower . IL-33 concentrations in the extracts were measured with a Quantikine ELISA kit (R&D Systems, Minneapolis, Minn) and read on a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, Calif). ZERO BIAS - scores, article reviews, protocol conditions and more. 5. 2. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Flow cytometry. This unit must survive. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing a single NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 5. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Data were fit by nonlinear regression to a one-site saturation binding model in Prism 5 (GraphPad Software). siRNA oligonucleotides targeted specifically to rat P2X 7 were used in this experiment. 5" H (50. Microplate Automation & Detection. Last lab had ours for years and never had a problem with it. For wavelengths in the UV range above 220 nm, UV-clear plastic plates or quartz plates should be used. Three kits covering a wide range of dsDNA concentrations. Cell growth was monitored by measuring OD values at 600 nm using a SpectraMax M5 plate reader (Molecular Devices, Silicon Valley, CA, United States). For experiments with TGX-221, the cells were treated with 200 nM of TGX-221 throughout the duration of the experiment. com 9. g. Wolfe1, and Stephen M. Abs). An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. Detection cartridge modularity allows for easy addition of new read modes within minutes without. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. I have a 2002 M5 and my medium-deluxe Harbor Freight reader doesn't talk to the BMW whatsoever. ScanLater Western Blot Detection Cartridge. For. The samples were loaded into a rectangular quartz cell with the light path length of 1 mm. The dual monochromator optics allow. Web Information The compact FLx800™ fluorescence reader provides high performance in 6- to 384-well microplates at an attractive price. The solids content was measured using a MA35 Moisture Analyzer (Sartorius AG). FeCl 3, iron standards are always included (0-4 µg /ml). 3. Cell Analysis. After addition of the freshly diluted substrate (50 μL/well, from Promega), bioluminescence was immediately measured on a SpectraMax M5 plate reader (Molecular Devices) using the luminescence mode. The fluorometric performance of the SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. 5: Schematic of the back panel of a reader. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. ". SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. Fluorescein 0.